Multilocus enzyme analysis of Legionella dumoffii

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Multilocus enzyme analysis of Legionella dumoffii.

Variability among 29 clinical and environmental strains of Legionella dumoffii was investigated by multilocus enzyme analysis by use of starch gel electrophoresis. Based on results of analysis at 20 enzyme loci, the strains were separated into five closely related electrophoretic types (ETs), which were clearly distinguished from 53 strains representing 53 ETs of L. pneumophila. DNA hybridizati...

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A new biotype of Legionella dumoffii.

A new biotype of L. dumoffii was isolated from lung and sputum samples of an immunosuppressed patient with pneumonia. This strain differs from other described strains of L. dumoffii in that it fails to produce browning of tyrosine-containing buffered yeast extract medium.

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Legionella dumoffii Utilizes Exogenous Choline for Phosphatidylcholine Synthesis

Phosphatidycholine (PC) is the major membrane-forming phospholipid in eukaryotes but it has been found in only a limited number of prokaryotes. Bacteria synthesize PC via the phospholipid N-methylation pathway (Pmt) or via the phosphatidylcholine synthase pathway (Pcs) or both. Here, we demonstrated that Legionella dumoffii has the ability to utilize exogenous choline for phosphatidylcholine (P...

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Detection of Legionella DNA in peripheral leukocytes, serum, and urine from a patient with pneumonia caused by Legionella dumoffii.

The polymerase chain reaction (PCR) has been used to detect Legionella DNA in respiratory tract, serum, and urine samples from patients with pneumonia. In addition, a preliminary study using a guinea pig model suggested that testing of peripheral leukocytes by PCR may be more sensitive than testing of other samples. We used PCR to detect Legionella DNA in serial peripheral leukocyte (buffy coat...

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Anti-Legionella dumoffii Activity of Galleria mellonella Defensin and Apolipophorin III

The gram-negative bacterium Legionella dumoffii is, beside Legionella pneumophila, an etiological agent of Legionnaires' disease, an atypical form of pneumonia. The aim of this study was to determine the antimicrobial activity of Galleria mellonella defense polypeptides against L. dumoffii. The extract of immune hemolymph, containing a mixture of defense peptides and proteins, exhibited a do...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1988

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.26.5.799-803.1988